The influence of biomass and species extracts of Ganoderma P. Karst on the seeds germination and the growth of Lepidium sativum L.

To determine the allelopathic effects of mycelial biomass of investigated strains was used a modified sandwich method: between two layers of agar medium was added 0.1 g of dry crushed biomass, followed by the introduction of seeds on the top layer. To determine the effect of methanol and ethyl acetate extracts, extract - soaked filter paper discs in sterile Petri dishes were used, to which 2 ml of sterile water was added after evaporation of the solvents, then seeds were added. Aqueous were adjusted to 2 ml with sterile water and impregnated the filter paper discs, after which the seeds were added. Biomass of all investigated strains shows allelopathic influence: the strongest - mycelium G. tsugae Murrill 2024 - inhibits plants growth by 80.9%, the weakest - G. carnosum Pat. 2502 - inhibits plants growth by 30.7% compared to control. Addition of 20 μl of G. sinense J.D. Zhao, L.W. Hsu & X.Q. Zhang 2516 aqueous extract stimulates shoot growth compared to the control by 19.3%. Ethyl acetate extracts of G. tsugae 2024 showed the highest allelopathic influence, which increased with increasing concentration of the extract - at a concentration of 100 μl, plant growth was inhibited by 100%.